what is stationary and mobile phase in chromatography
Answers
Explanation:
They mean exactly what you would expect. Take as your model paper chromatography. You place a spot of black ink 1cm from the bottom of the paper strip. Then dip the paper into a suitable solvent. This soaks up the paper and sweeps the ink spot upwards. As it travels across the paper the different colour components of the ink separate into different individual colours. At the end of the run when the solvent has migrated to near the top of the page you may have a green, red and blue spot.
In this model the
paper is the stationary phase, because it stays still
solvent is the mobile phase because it moves
the sample is the black ink spot
application is by blotting the ink on the paper
we detect by viewing the differnet colour spots with our eyes
This model applies to all chromatography techniques but each component changes.
For example HPLC
modified silica packed in a metal cylinder (column) is the stationary phase
solvent pumped through the column is mobile phase, whose composition can changed by using a gradient former
the sample is a solution of your mixture of compounds
application to the stationary phase is by six port injection valve into the flow path
the detection is UV absorption generating peaks on a chromatogram.
Extensive reading on the subject will further explain