what Is the advantage of 2d gel electrophoresis over 1d gel electrophoresis??
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2 Dimensional gel electrophoresis :-
In 1975 a technique called two - dimensional gel electrophoresis was developed by Patrick O Farrel at the University of California San Francisco to fractionate complex mixture of proteins are first separate in a tubular gel according to their isolateteric point by a technique called and placed on top of a slab of SDS structured polyacrylamide and subject to SDS - page .
The protein move into the slab gel and become separated according to their molecular proteins can be removed gel and digested into peptide fragments that can be analysed by mass spectrometry.
The resolution of the technique is sufficiently high to distinguish most of the proteins in a cell.
Because of its great resolving power two dimensional gel electrophoresis is ideally suited to detect Change in the proteins present in a cell under different conditions, at different stages in development or the cell cycle or in different organisms.
Polycrylamide gel electrophoresis :-
The protein sample are typically dissolved in a sucrose in a solution whose density prevents the sample from mixing with the buffer and than loaded into the wells with a fine pipette as shown in step 1, in step 2 a direct current is applied across the gel which causes the protein to move into the polycrylamide along parallel lanes l.
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I hope it's help you... !!! :)
In 1975 a technique called two - dimensional gel electrophoresis was developed by Patrick O Farrel at the University of California San Francisco to fractionate complex mixture of proteins are first separate in a tubular gel according to their isolateteric point by a technique called and placed on top of a slab of SDS structured polyacrylamide and subject to SDS - page .
The protein move into the slab gel and become separated according to their molecular proteins can be removed gel and digested into peptide fragments that can be analysed by mass spectrometry.
The resolution of the technique is sufficiently high to distinguish most of the proteins in a cell.
Because of its great resolving power two dimensional gel electrophoresis is ideally suited to detect Change in the proteins present in a cell under different conditions, at different stages in development or the cell cycle or in different organisms.
Polycrylamide gel electrophoresis :-
The protein sample are typically dissolved in a sucrose in a solution whose density prevents the sample from mixing with the buffer and than loaded into the wells with a fine pipette as shown in step 1, in step 2 a direct current is applied across the gel which causes the protein to move into the polycrylamide along parallel lanes l.
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I hope it's help you... !!! :)
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