Question

What is the advantage of using shuttle vector over cosmid vector?

Answer 1

Answer:

SHUTTLE VECTOR:

• A shuttle vector is a vector designed to propagate in exceptional host species. Therefore, overseas DNA inserted right into a shuttle vector may be examined or manipulated in exceptional cell types.
• Normally, a  shuttle vector has origins of replications, every of that's unique to a host. As go back and forth vectors reflect in exceptional hosts, they're additionally referred to as bifunctional vectors. One famous shuttle vector is the yeast shuttle vector.
• nearly all of the generally used Saccharomyces cerevisiae vectors are shuttle vectors. For example, the yeast shuttle vector incorporates additives that permit replication and choice in each yeast cell in addition to E. coli cells. The E.coli additives of the yeast shuttle vector are a foundation of replication and a selectable marker (e.g., antibiotic resistance, beta-lactamase).

COSMID VECTOR:

• Cosmid is a round as DNA
• It has complementary single-stranded areas at each end of a plasmid DNA. The cos-ends shape a duplex through base pairing.
• The cosmid DNA does now no longer code for phage proteins and host molecular lysis.
• It does now no longer contain in, the multiplication of phage particles.
• It has a foundation of replication from plasmid DNA for unbiased replication.
• It has selectable marker genes and gene cloning websites of plasmid DNA
• The cosmid DNA is packed inside the protein coat of bacteriophage to shape inactive phage particles.
• cosmid is a prerequisite for invitro packaging of cosmid in a phage protein coat. After infection, the cosmid DNA does now no longer combine into host chromosomal DNA. It exists as a definite
• greater chromosomal DNA and replicates independently.

these are some of the reasons why shuttle vector over cosmid vector!

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Answer 2

Answer:

The shuttle vector is used for propagating the E.coli and mammals.

Step-by-step explanation:

• A shuttle vector includes the plasmids which can propagate in the eukaryotes and prokaryotes.
• The different species of bacteria such as E.coli and Rhodococcus erythropolis it propagates with the E.coli and mammals. It is known as adenvorius shuttle vectors.

• Characteristics of the shutter vectors are:

They are constructed and the characteristics that are basic it will include efficiency of transformation, plasmid copy number and plasmid stability.

• Uses of the shuttle vector:

It is used for quickly multiplying the copies of a gene in amplification process of the E.coli.

It is used for invitro experiments and other kinds of modification of CR and mutagenisis.

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