Question

what is the artificial medium used in tissue culture????????????

Answer 1

The essential elements in a plant cell or tissue culture media include, besides C, H and O, macroelements: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg) and sulphur (S) for satisfactory growth and morphogenesis. Culture media should contain at least 25-60 mM of inorganic nitrogen for satisfactory plant cell growth. Potassium is required for cell growth of most plant species. Most media contain K in the form of nitrate chloride salts at concentrations ranging between 20 and 30 mM. The optimum concentrations of P, Mg, S and Ca range from 1-3 mM if other requirements for cell growth are provided