What is the basic principle of centrifugation method in short
Answers
Explanation:
A centrifuge works by using the principle of sedimentation: Under the influence of gravitational force (g-force), substances separate according to their density. Different types of separation are known, including isopycnic, ultrafiltration, density gradient, phase separation, and pelleting.
Answer:
A centrifuge works by using the principle of sedimentation: Under the influence of gravitational force (g-force), substances separate according to their density. Different types of separation are known, including isopycnic, ultrafiltration, density gradient, phase separation, and pelleting.
In general, applications for centrifugation specify the degree of acceleration to be applied to the sample rather than specifying a specific rotational speed such as revolutions per minute. The acceleration is typically given in gravity [× g] (or multiples of x g or g-force), the standard acceleration value due to gravity at the Earth’s surface (9.81 m/s2). The distinction between rpm and rcf is important, as two rotors with different diameters running at the same rotational speed (rpm) will result in different accelerations (rcf).