what is the basics of separation of different DNA fragments by gel electrophoresis
Answers
Answer:
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Explanation:
A basic protocol for the separation of DNA fragments using agarose gel
Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. ... This treatment makes the proteins unfold into a linear shape and coats them with a negative charge, which allows them to migrate toward the positive end of the gel and be separated.
process
1. Understand the mechanism by which DNA fragments are separated within a gel matrix
2. Understand how conformation of the DNA molecule will determine its mobility through a gel matrix
3. Identify an agarose solution of appropriate concentration for their needs
4. Prepare an agarose gel for electrophoresis of DNA samples
5. Set up the gel electrophoresis apparatus and power supply
6. Select an appropriate voltage for the separation of DNA fragments
7. Understand the mechanism by which ethidium bromide allows for the visualization of DNA bands
8. Determine the sizes of separated DNA fragments