Question

What is the correct order of staining reagents in Gram-Staining?

a) Crystal violet, alcohol, iodine solution, safranin

b) Crystal violet, iodine solution, alcohol, safranin

c) Crystal violet, safranin, alcohol, iodine solution

d) Iodine solution, crystal violet, alcohol, safranin​

Answer 1

Answer:

answer is d

iodine solution:crtal violet:alcohol: safranin

Answer 2

Option b) The correct order of staining reagents in Gram-Staining is Crystal violet, iodine solution, alcohol, safranin.

• Crystal violet is the primary stain that is used to stain the bacterial smear on the slide.
• Iodine, which is a mordant, supports the adherence of the violet color to the cell wall.
• Alcohol is a decolorizer that is used to wash off the color from unstained cells.
• Safranin is the secondary stain that stains those cells that could not take the violet dye.
• It is observed that the bacteria that take up the violet dye are gram-positive and the cells that take safranin dye are gram-negative dye.