Question

what is the detection technique of auxotroph​

Answer 1

Answer:

This technique is used to detect auxotrophic mutants which differentiates between mutants and wild type strains on the basis of ability to grow in the absence of an amino acid. ... The leucine auxotrophs (Leu–) will not grow on replica plates devoid of leucine. Isolate and culture Leu– cells growing on complete medium.

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