Question

What is the difference between collision and rolling collision energy in mass spectrometry?

Answer 1

This video describes a short optimization project to measure the optimal collision energy curves for TripleTOF systems, for performing IDA and SWATH experiments. Rolling collision energy curves were measured on 3 different instruments and then validated on additional instruments. Gains of 5-10% in peptide/protein IDs were found with the new curves. The new curves are compatible with the 100 variable window SWATH method and the Collision Energy Spread (CES) observations are already built into this VW method.