What is the difference between dna polymerase 1 and 3?
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Polymerase 1 removes the RNA primer and replaces with a nucleotide. Polymerase 3 helps in extension of chain of nucleotide from RNA primer. Both act during DNA replication.
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Two molecules of DNA polymerase III bind to the primers on the leading and lagging strands and synthesize new DNA from the 3′ hydroxyls (Fig. 4.5). DNA polymerase cannot synthesize new DNA without a pre-existing 3′-OH. Thus, DNA replication requires an RNA primer to initiate strand formation.
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