Question

What is the difference between thin layer chromatography and paper chromatography?

Answer 1

Answer:

Difference Between Thin Layer Chromatography And Paper Chromatography

Thin layer chromatography:

1. TLC is a chromatography technique used to separate non-volatile mixtures.
2. It is performed on a sheet of glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide, or cellulose.
3. It can be used to monitor the progress of a reaction, identify compounds present in a given mixture.
4. TLC plates can be heated to higher temperatures without any damage.

Paper chromatography:

1. Paper chromatography is an analytical method.
2. The paper is made of cellulose, a polar substance, and the compounds within the mixture travel farther if they are non-polar.
3. It is used to separate coloured chemicals or substance, and also in secondary or primary colours in ink experiments.
4. If the paper chromatography is heated to higher temperatures, the paper gets damaged.

Answer 2

Explanation:

Paper chromatography requires less preparation whereas thin layer chromatography requires more preparation time. The stationary phase of paper chromatography is the water trapped in the cellulose filter paper. ... Thin-layer chromatography makes the use of silica gel whereas paper chromatography does not.