what is the effect of overheating a medium during sterilization by autoclaving
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Explanation:
overheating is a common cause of pH drift, darkening, precipitation, poor gel strength and reduced bacteriological performance
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Overheating of medium by autoclaving will result in poor gel formation in a solid medium, precipitation, darkening of medium and poor growth of the microorganisms.
Explanation:
- All the culture media must be in solution before sterilisation to reduce the non-enzymatic browning takes place in the medium.
- Sterilisation is an important method in the study of microbes in which the unwanted growth of other microorganisms in the culture media is prohibited.
- The culture medium is a special medium used in microbiological labs to grow the desired microorganisms.
- The culture medium contains the nutrients which are used by microbes for their growth.
- An autoclave is a machine that works under pressure to sterilize the solids, liquids, glassware, and instruments.
- To sterilize the cultural medium, Autoclave uses saturated steam at 121 degrees Celsius temperature, 15 psi pressure for 15 -20 minutes.
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