Question

What is the first step in the Southern Blot technique [AIIMS 2004]
A) Denaturation of DNA on the gel for hybridization with specific probe
B) Production of a group of genetically identical cells
C) Digestion of DNA by restriction enzyme
D) Isolation of DNA from a nucleated cell such as the one from the scene of crime

Answer 1

hey hi...

the correct answer is option D.



>>>The first step in southern blot technique is isolation of DNA from a nucleated cell and then these isolated DNA sequences are transferred to a nylon or nitrocellulose sheet placed over the gel....

hope this helps....

Answer 2

What is the first step in the Southern Blot technique [AIIMS 2004]
A) Denaturation of DNA on the gel for hybridization with specific probe
B) Production of a group of genetically identical cells
C) Digestion of DNA by restriction enzyme
D) Isolation of DNA from a nucleated cell such as the one from the scene of crime✔✔✔