Question

What is the function of EDTA in DNA extraction?

Answer 1

Explanation:

However, if you're talking about DNA extraction, EDTA is a chelating agent used to remove DNAse from the environment after the nuclear membrane has been broken down. EDTA has an affinity for Mg ions; so it binds the Mg and makes it unavailable to the enzymes, rendering them inactive

Answer 2

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ANSWER ==

EDTA act as cation chelating agent. hence it is used in DNA isolation to chelate the Mg++ ions which are necessary in activity of nucleases so your DNA will be safe in presence of EDTA. ... After the nuclear membrane has broken, the EDTA is used to remove DNase from the environment.

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