Question

What is the importance of deducing the isoelectric point of an amino acid?

Answer 1

The isoelectric point (pI) is the pH value at which the molecule carries no electrical charge. The concept is particularly important for zwitterionic molecules such as amino acids, peptides, and proteins.

Answer 2

Explanation:

The isoelectric point is significant in protein purification because it represents the pH where solubility is typically minimal. Here, the protein isoelectric point signifies where mobility in an electro-focusing system is zero—and, in turn, the point where the protein will collect.