what is the isolation of water
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I'd do an enrichment of the surface water first before trying to culture it on a plate. If you try to culture directly onto a plate you may lose a significant portion of the organisms who just can't survive on the typical medium - that whole "Great Plate Count Anomaly" thing.
An enrichment should be relatively simple out of pond/lake water. A small sample of river water, plus maybe some DI water, supplemented with the pharmaceuticals of your choice and leave it out at room temperature or in an incubator for several days. Then you can do some FISH, or some 16S amplification on the sample to get clades of relatedness. Once you have clades/identities through the sequencing, you can probably more reliably find a way to culture it in vitro. Though there is always the chance that anything you find may not be culturable in the lab.
2 R
An enrichment should be relatively simple out of pond/lake water. A small sample of river water, plus maybe some DI water, supplemented with the pharmaceuticals of your choice and leave it out at room temperature or in an incubator for several days. Then you can do some FISH, or some 16S amplification on the sample to get clades of relatedness. Once you have clades/identities through the sequencing, you can probably more reliably find a way to culture it in vitro. Though there is always the chance that anything you find may not be culturable in the lab.
2 R
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