What is the material required for permanent staining?
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The material after thorough washing is passed through the gradually increasing percentage of the medium in which stain is made e.g., since the borax carmine or picro-indigo-carmines are usually made in 70% alcohol the material should first be passed through 30% alcohol, 50% alcohol and 70% alcohol for at least 3 ...
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Wet mounts suspend specimens in fluids such as water, brine, glycerin and immersion oil.
Staining solutions such as iodine, methylene blue and crystal violet can be added to wet or dry mounts.
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