What is the meaning of template independent manner
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Answer:
Polynucleotide phosphorylase
Explanation:
tailing occurs by adding adenylate residues at 3’ end by poly A polymerase enzyme...this enzyme helps in RNA synthesis without the use of template strand...so tailing is done in template independent manner...hope this ans helps u
additional info
Polynucleotide phosphorylase polymerizes RNA with defined sequence in a template independent manner. PNPase is a bifunctional enzyme with a phosphorolytic 3' to 5' exoribonuclease activity and a 3'-terminal oligonucleotide polymerase activity.
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