Question

what is the meant by cryopreservation

Answer 1

Cryopreservation or cryoconservation is a process where organelles, cells, tissues,extracellular matrix, organs or any other biological constructs susceptible to damage caused by unregulated chemical kinetics are preserved by cooling to very lowtemperatures[1] (typically -80 °C using solidcarbon dioxide or -196 °C using liquid nitrogen). At low enough temperatures, anyenzymatic or chemical activity which might cause damage to the biological material in question is effectively stopped. Cryopreservation methods seek to reach low temperatures without causing additional damage caused by the formation of ice during freezing. Traditional cryopreservation has relied on coating the material to be frozen with a class of molecules termedcryoprotectants. New methods are constantly being investigated due to the inherent toxicity of many cryoprotectants.[2] By default it should be considered that cryopreservation alters or compromises the structure and function of cells unless it is proven otherwise for a particular cell population.Cryoconservation of animal genetic resourcesis the process in which animal genetic material is collected and stored with the intention of conservation of the breed.