what is the mechanism of muroxide tests
Answers
Answer:
The murexide test for uric acid consists in the reaction of uric acid with dilute nitric acid, followed by ammonia addition: a purple colouration is produced due to the formation of ammonium purpurate or murexide. The reaction mixture is heated to dryness in a porcelain dish on the water bath.
Explanation:
The murexide test is an analytical technique to identify the presence of caffeine and other purine derivatives in a sample. These compounds do not respond to the common alkaloid detecting tests such as Dragendorff's reagent. In this test the alkaloids are mixed with a tiny amount of potassium chlorate and a drop of hydrochloric acid. The sample is then evaporated to dryness and the resulting residue is exposed to ammonia vapour. Purine alkaloids produce a pink colour in this test. Murexide (ammonium purpurate) with a purple colour is also produced in this test