Question

What is the order of reagents used in the Gram stain?
A.Crystal violet, iodine, safranin, decolorizer
B. Crystal violet, iodine, decolorizer, safranin
C. Safranin, crystal violet, decolorizer, iodine
D. Crystal violet, safranin, iodine, decolorizer

Answer 1

Heya!

The answer will be B)

1. stained with Crystal violet.

2. Iodine staining (used as mordant)

3. Treated with alcohol

4. gram -ve bacteria counter stained with safranin

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Hope this helped u..