Question

what is the precautions take to prepare a temporary amount of a leaf peel to show its stomata.

Answer 1

1. Put the cover slip carefully, don't make hurry to put it.
2. Moisten the peel with glycerin before it gets dried.
3. Proper amount of light should be provided to the microscope.
4. Safranin should be properly used to stain the peel.

Answer 2

1. Cut the peel to a proper size and avoid folding it.

2. Always place the peel at the centre of the slide and hold the slide at the edges.

3. Do not overstrain or under strain the peel.

4. Always handle the peel with a brush as a needle may damage the cells.

5. Take care to prevent the peel from drying by using glycerin.

6. Place the coverslip gently, avoiding any air bubbles.

7. Remove excess stain and glycerin with a blotting paper.