what is the principle involved in estimation of the urea nitrogen in urine by dam method
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Principle Urea in an acidic medium condenses with Diacetyl monoxime at 100°C to form a red coloured complex. Intensity of the colour formed is directly proportional to the amount of urea present in the sample. Mix well and keep the test tubes in boiling water (100°C) for 10 minutes.
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