Question

What is the principle of chromatography???

Pls help

ASAP

Answer 1

It is defined as the process of separation of the individual components of a mixture based on their relative affinities towards stationary and mobile phases. Principle: The samples are subjected to flow by mobile liquid onto or through the stable stationary phase.

Answer 2

Chromatography is a non-destructive procedure for resolving a complex mixture into its individual fractions or compounds. This is a separation procedure and the separated entities are identified by other analytical techniques like UV-visible, Infrared, NMR (nuclear magnetic resonance), Mass spectroscopy etc. For quantitative analysis the measurement of the area under the curve in chromatogram is done.

"Chromato" "graphy" derives its name from two words as chromo= colour and graphy= writing.

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