Question

what is the principle of Flourimetry​

Answer 1

Answer:

Fluorescence is the phenomenon of emission of radiation when there is transition from singlet excited state to singlet ground state.  Wavelength of absorbed – Excitation wavelength.  Wavelength of emitted – Emission wavelength.  These two are specific for a given substance under ideal condition

Answer 2

Answer:

intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. These parameters are used to identify the presence and the amount of specific molecules in a medium.