What is the procedure to prepare the temporary mount of leaf peelto observe the stomata?
Answers
Remove a healthy leaf from the potted plant.
2. Remove a part of the peel from the lower surface of the leaf. You can do this by folding the leaf over and gently pulling the peel apart using forceps. Keeps the peel in a watch glass containing water.
3. Put a few drops of safranin stain in a watch glass.
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4. After 2-3 minutes take out the peel and place it on a clean glass slide.
5. Put a drop of glycerin over the peel and place a clean coverslip gently over it with the help of a needle.
6. Remove the excess stain and glycerin with the help of blotting paper.
7. Observe the slide under the low-power and high-power magnifications of the compound microscope.
Explanation:
The following are the steps ofpreparation of temporary amount of aleaf peel to observe stomata. (i) Remove a healthy leaf from the potted plant. (ii) Remove a part of the peelfrom the lower surface of the leaf by folding the leaf over and gently pulling the peel apart using forceps.