what is the purpose of softening the leaf by boiling in water
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This procedure kills a leaf, disrupts the cell membranes and softens the cuticle and cell walls. This makes it possible to extract the chlorophyll with hot ethanol and also allows the iodine solution to penetrate the cells and react with any starch present.
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A leaf is killed, the cell membranes are disrupted, and the cuticle and cell walls are softened during this procedure. This causes the chlorophyll to be extracted with hot ethanol, as well as the iodine solution to enter the cells and react with any present starch.
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