What is the ratio between the distance travelled by a spot and the distance travelled by the solvent front during paper chromatography known as?
Answers
Answer:
Paper chromatography is an analytical method used to separate colored chemicals or substances.[1] It is primarily used as a teaching tool, having been replaced by other chromatography methods, such as thin-layer chromatography. A paper chromatography variant, two-dimensional chromatography involves using two solvents and rotating the paper 90° in between. This is useful for separating complex mixtures of compounds having similar polarity, for example, amino acids. The setup has three components. The mobile phase is a solution that travels up the stationary phase, due to capillary action. The mobile phase is generally mixture of non-polar organic solvent, while the stationary phase is polar inorganic solvent water. Here paper is used to support the stationary phase, water. Polar water molecules are held inside the void space of cellulose network of the host paper. Difference between TLC and paper chromatography is that stationary phase in TLC is a layer of adsorbent (usually silica gel, or aluminium oxide), and stationary phase in paper chromatography is water.
Explanation:
The Rf value is defined as the ratio of the distance moved by the solute (i.e. the dye or pigment under test) and the distance moved by the the solvent (known as the Solvent front) along the paper, where both distances are measured from the common Origin or Application Baseline, that is the point where the sample is ...