Question

What is the reaction in DNA replication catalysed by DNA ligase ?
1) Addition of new nucleotides to the leading strand
2) Addition of new nucleotide to the lagging strand
3) Formation of a phosphodiester bond between the 31 - OH of one Okazaki
fragment and 5! - phosphate of the next on the lagging strand
4) Base pairing of the template and the newly formed DNA strand.​

Answer 1

Answer:

The reaction in DNA replication that is catalyzed by DNA ligase is attaching the 3' end of one nucleotide to the 5' end of the next one..

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Answer 2

Answer:

The correct option is 3)

Explanation:

• The joining of Okazaki fragments requires an enzyme DNA ligase that catalyzes the joining of the ends of two DNA chains. This enzyme catalyzes the formation of a phosphodiester bond between the 3'- OH at the end of one DNA chain and the 5'- phosphate group at the end of the other.
• An energy source is required to drive this process. In eukaryotes and archaea, ATP is the energy source. In eubacteria, NAD⁺ typically plays this role. ATP or NAD⁺ functions as the AMP donor during the reaction catalyzed by DNA ligase and PPi or NMN is released.

Hence, the correct option is 3)