Question

What is the role of bioreactor in recombinant DNA technology ?

Answer 1

Bio reactor is an apparatus in which a biological reaction or process is carried out. The chemical reactions take place in presence of microorganisms in this bioreactor. It can be aerobic or anaerobic. Sterile air (oxygen bubble) is poured inside a bioreactor to prevent the cake like layer of biomass over the liquid. Biorecator helps to mix the nutrients well with water and keeps the fermentation homogeneous.

Answer 2

Dear Student,

◆ Role of bioreactor in rDNA technology -

• Bioreactor provides necessary conditions for cultre of host organism
• It helps in large scale cultivation of rDNA

◆ Steps in rDNA technology -

• Isolate vector plasmid and human DNA
• Insert human DNA into plasmid
• Put plasmids into host bacteria by transformation
• Multiplication of rDNA in bioreactor to produce many copies
• Identify clone carrying DNA of interest

Thanks dear. Hope this is helpful to you ..