Question

What is the role of cuso4 and k2so4 in the estimation of nitrogen content of any sample by kjeldahl method?

Answer 1

Analytical balance, sensitive to 0.1 mg

Reagents:
Sulfuric acid, concentrated, 95-98%, reagent grade Sodium hydroxide, pellets, flakes, or 45% solution with specific gravity �1.36 (low N) dissolve 450 g in cool water and dilute to 1 L Potassium sulfate (K2SO4), anhydrous Copper sulfate (CuSO4), anhydrous Titanium dioxide (TiO2) Alundum, boiling stones, 8-14 mesh Pumice Methyl red indicator dissolve 1 g methyl red (sodium salt) in 100 mL methanol or ethanol Tributyl citrate (for antifoam) or paraffin or antifoam A or equivalent Lysine monohydrochloride, reagent grade, dried at 110OC for four hr Hydrochloric acid standard solution, 0.5 N Prepare by diluting 430.1 mL 36.5 

Answer 2

Hey dear,

● Answer -
- Copper sulphate and potassium sulphate are used as catalysts in nitrogen estimation by Kjeldahl method.

● Kjeldahl method -
- Kjeldahl method is used for estimation of nitrogen content in given organic/inorganic sample.

This involves 3 simple steps -
1) digestion - organic nitrogen is converted to NH4+.
2) distillation - NH3 is distilled and retained in reciever vessel.
3) Titration - nitrogen is determined



Hope this helps you...