Question

what is the role of Janus green B stain in this slide preparation
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Answer 1

Janus green B is a green colour dye which is basic in nature.

Explanation:

• it is used in the molecular structure for determination of the contents of the nucleus of a particular cell or its DNA sequence. This is because the DNA consists of nucleic acid which is acidic in nature. It picks up the basic dye.
• This results in the formation of a acid and a base resulting in a different colour which is green. Hens on different amount of the green colour there is determination of the amount of DNA or the amount of nucleic acid which is present in a cell

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