What is the significance of boiling the leaf in ethanol while testing it for the presence of starch?
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We boil the leaf in alcohol when we are testing it for starch because to remove the green pigment chlorophyll present in it. For the starch test we need to observe the color change from brown to blue when iodine is put on the leaf.
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This procedure kills a leaf, disrupts the cell membranes and softens the cuticle and cell walls. This makes it possible to extract the chlorophyll with hot ethanol and also allows the iodine solution to penetrate the cells and react with any starch pre
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