What is the use of DNA polymerase, DNA ligase, endonucleas?
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Endonuclease - cleavage of DNA at specific base sequence
Ligase- Joins DNA molecules or fragments
DNA polymerase- fills gap after removal of RNA primer,DNA repair
Ligase- Joins DNA molecules or fragments
DNA polymerase- fills gap after removal of RNA primer,DNA repair
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DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA. In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids.
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