what is the use of PTK and PTD ENZYME in cell culture
Answers
Explanation:
Trypsin acts by cutting amino acids, specifically lysines or arginies, on their c-termini unless these amino acids are followed by an proline. In cell culture, the way I understand it is that you are cutting away the focal adhesions that are anchoring the cell to the culture dish. Most trypsin solutions for cell culture also contain EDTA which acts as a chelator for calcium. By removing calcium from a solution with cells, cadherins which hold cells to each other, are broken and cells separate from each other as well as from the surface of the tissue culture plastic.
Answer:
Gibco media, FBS & reagents offer a reliable solution for all your cell culture needs. Manufactured at a cGMP-compliant facility for superior quality, consistency & control.