what is tissue culture what are steps involved in this
Answers
Answer:
Tissue culture is a technique in which fragments of plants are cultured and grown in a laboratory. Many times the organs are also used for tissue culture. The media used for the growth of the culture is broth and agar. This technique is also known as micropropagation.
Steps in tissue culture technique -
1) Selection of mother plant
2) Initiation of culture
3) Multiplication
4) Root formation
5) Transfer to soil
Applications of tissue culture -
1) Early flowering
2) Synthetic seeds
3) Fast multiplication
4) Genetic transformation
5) Clonal propagation
6) Disease prevention
7) Large scale multiplication
8) Self-sterlity is overcame
9) Viability of seeds improved
10) Hybridization with wide variety of plants
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Answer:
The steps are: 1. Inoculation of Explant 2. Incubation of Culture 3. Sub-Culturing 4. Transplantation of the Regenerated Plant.
Explanation:
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