Question

What is used while preparing a temporary mount of human cheek cell and why?

Answer 1

Methylene blue stain and glycerin are the main compounds that are used.

Methylene blue will produce a darker stain in areas where those components are present so that it can be clearly seen in a light microscope. Glycerin is used

1. it is a pretty good dehydrating agent

2. Due to its refractive nature it tends to reflect the light better and as a consequence the image is clearer and better under microscope- observation.

3. transparent so image is better

4. easy to slide a cover slip on a glycerin drop

and others .....

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