What is vif?what r effect in human
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The Vif protein of human immunodeficiency virus type 1 (HIV-1) is
important for virion infectivity. Previous studies have shown that vif
mutant HIV-1 virions are defective in their ability to synthesize
proviral DNA in vivo. Here, we examine the role of Vif in viral DNA
synthesis in the endogenous reverse transcriptase (RT) reaction, an in
vitro assay in which virions synthesize viral DNA by using endogenous
viral RNA as a template. vif mutant virions showed a significant
reduction in endogenous RT activity despite similar levels of exogenous
RT activity. Analysis of the viral DNA products on agarose gels demonstrated that
this reflects reduced synthesis of short minus- and plus-strand DNA
products in addition to those of full genomic length. Quantitative PCR
analysis of endogenous reverse transcription provided further evidence
for reduced formation of both initial and completed reverse transcripts.
Vif had no effect on genomic RNA dimerization or the stability of the
RNA dimer linkage. These results suggest that Vif is important for an
early event after virus entry but preceding or during the early stages
of viral DNA synthesis. This may be due to an intrinsic effect on
reverse transcription or a preceding postentry event(s), such as virion
uncoating or disassembly of the virion core. Drugs targeted to Vif
function may provide a new therapeutic approach to inhibiting HIV-1
reverse transcription.
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The Vif protein of human immunodeficiency virus type 1 (HIV-1) is important for virion infectivity.
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