Question

What media can be used to isolate anerobic thermophiles?

Answer 1

Answer:

Don't complicate matters too much if you are a beginner to thermophile research. Dilute Nutrient broth (0.1%) or dilute Trypticase Soy Broth (0.1%) is a good complex medium to start off with. TSB can be pHed to alkaline pH if required.

If you need to isolate colonies than 2.5% agar can be added.However make sure that you autoclave the medium for at least 30 mins. Agar is loaded with spore from thermophilic bacilli and they grow as contaminants if you fail to autoclave the medium.

To avoid water condensation on agar surface, you should try to dry the plates though this may not be entirely necessary.

Culturing anaerobes is another matter ............