What precautions should be taken while preparing onion root tip squash preparation to observe mitosis stages ?
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Explanation:
1. Lab Procedure:
Take an onion and place it on the tile.
Carefully remove the dry roots present using a sharp blade.
Grow root tips by placing the bulbs in a beaker filled with water.
New roots may take 3–6 days to grow.
Cut off 2–3 cm of freshly grown roots and let them drop into a watch glass.
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ANSWER
Procedure
Take the onion plant with newly sprouted roots and cut two root tips using scissors and transfer them into a plastic microfuge tube.
Fill 2/3 of the tube with 1N HCl using a dropper.
Place the tube in a 60°C water bath and incubate the tube for 12- 15 minutes.
Remove the tube from the water bath after the incubation.
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