What procedure we follow to observe fungi
Answers
Explanation:
Is there anybody working on fungus? I am facing a problem in identification of fungus under microscope. I use lactophenol cotton blue flooding over slide where fungus is placed from fungal plate. Then i use cover slip on it. But the mycellium is not clear under microscope, i mean it shows a bizarre condensed growth.
Answer:
Two methods you can use first slide culture you put cover slip on fungi colony then take it with care and safety considerations use of mask and bio safety cabinets put on glass slide put a drop methylene blue from slide margin you can watch directly mycelium structure with its spore under microscope in 10.
Second you must take a sample of colony fix it by alcohol and then staining it but may be you can watch a portion of mycelium and spore in fixed condition but in mycology safrty issues must be consider seriously because of opportunistic fungi risk.
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