What procedure you follow in your school laboratory to observe the nucleus in cheek cells?what precautions you should take while doing experiment?
Answers
Procedure
1.wash your mouth and scrape a little of the internal winning of your cheek inside your mouth with a tooth pick.
2.place the scraped material in a watch glass containing a very small quantity of normal saline.
3.then place the material on a glass slide.
4. Put a drop of ethylene blue and wait for a couple of mins.
5.Put a drop of glycerineover it.
6.place a coverslip.tap the coverslip with the blunt end of needle so as to spread the cells.
7.wipe off the extra stain with a fine cloth or blotting paper.
Precautions
Do not scrape the cheek too hard as it may injure your buffalo muscle
Excess stains should be drained off.
There should be no air bubbles under the cover slip.
PRECAUTIONS :-
. ♦ do not scrap the cheek too hard as it may cause injury
. ♦ excess stain should be [taken] drained off.
. ♦ care should be taken to avoid air bubbles under the cover slip.
. ♦ scrapped material should be spread uniformly on the slide.
. ♦discard the toothpick properly after scrapping.