Question

What role are the following play in cloning a DNA fragment?
a.restriction enzymes
b.vectors
c.DNA ligases
d.electroporation

Answer 1

a) restriction enzyme cut the DNA but in a specific location so clled as restriction endonuclease
b) Vectors... like a bacteria it contain plasmid...than cut DNA is introduce in the host host cell with the help of plasmid(vector)
3) DNA ligase is linking the cut DNA or foreign to plasmind..
4) restrictions endonuclease cut the recombinant DNA into small fragments than use gel electroporasis machine small partical of DNA comes in a first row ND large in the back row ND this DNA molecule attract towards plate ve+ end ND uh know DNA is a ve+ molecules Bcz of phosphate group
...so small piece of DNA attract first as compare to large DNA ND the gel present in this process is Across gel which can provide by the agar agar jelly which provide by the bacteria