What roles do the following play in cloning a DNA fragment?
(a) Restriction enzymes.
(b) Vectors.
(c) DNA ligase.
(d) Electroporation.
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A gene or other piece of DNA can be cloned by cutting the DNA from its source and then pasting it into a DNA vector that helps to replicate it.
Restriction enzymes: help in cloning by cutting DNA at specific 4- to 8-bp sequences
Vectors: DNA can be cloned by injecting it into the vector DNA
DNA ligase: used to insert genes and other pieces of DNA into the plasmids to clone DNA fragment
Electroporation: used to transfer the foreign DNA into the fragile cells with the help of electric transfer
Restriction enzymes: help in cloning by cutting DNA at specific 4- to 8-bp sequences
Vectors: DNA can be cloned by injecting it into the vector DNA
DNA ligase: used to insert genes and other pieces of DNA into the plasmids to clone DNA fragment
Electroporation: used to transfer the foreign DNA into the fragile cells with the help of electric transfer
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