Question

what roles do the following play in cloning a DNA fragment ( a)ristriction enzymes (b) vector (c) dna ligase (d) electroporation

Answer 1

(a) restriction enzyme helps in cutting of desired DNA fragment from the whole DNA strand

(b)vector helps in transmission of the desired DNA fragment or foreign DNA material into other cell where it can be replicated

(c)DNA ligase helps in attaching the DNA strands by formation of phosphodiester bonds

(d)process in which membrane is made permeable for allowing chemicals,drugs,or DNA which is to be introduced by applying electric field to cells is called as electroporation..