Question

What roles do the following play in cloning a DNA fragment?

(a) Restriction enzymes.

(b) Vectors.

(c) DNA ligase.

Answer 1

A-Restriction enzymes are very much necessary for cutting of DNA strands at specific sequence so that we can add a new gene into it example would be restriction endonuclease  B- vectors are very much needed for the transfer of gene that we want to insert or modify example would be cosmid  C- DNA ligase is necessary for the binding of DNA molecules after they have been deattached using restriction endonuclease as it helps in binding phosophodiester bonds

Answer 2

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