Biology, asked by njhambh6064, 1 year ago

What should be concentration of protein in a ml, while estimating protein?

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Answered by darshu2861
0

Answer:

Use the following formula to roughly estimate protein concentration. Path length for most spectrometers is 1 cm. Concentration (mg/ml) = Absorbance at 280 nm divided by path length (cm.) Pure protein of known absorbance coefficient.

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