Biology, asked by tshering8257, 1 year ago

What should be rna concentration for cdna synthesis?

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Answered by srilakshmi23

Most protocols suggest to use between 0.1 to 1 ug RNA. Up to 5 ug can be used but I am not sure about it. ... For RNA to cDNA synthesis, we use 1 ug of RNA, then the final (20 ul) reaction mix is added to 80 ul of diH2O (final cDNA concentration 1:5), and our data is beautfiful for qPCR / rt-PCR.

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