Question

What should be the lambda max of cu edta in sodium acetate buffer?

Answer 1

A developed, direct, rapid, and sensitive spectrophotometric determination of beryllium in beryl minerals with Chrome Azurol S (CAS) as a chromogenic reagent was developed in the present article. The determination was performed without either solvent extraction or ion exchange separation of beryllium from its matrix. The stable 1:1 Be-CAS complex was formed instantly with duration time of at least 24 h with constant absorbance. Different parameters such as wavelengths, pH, EDTA concentration and dye concentration were studied for the optimum determination of beryllium. Interference due to presence of high aluminum concentrations was overcome by adding 5% EDTA disodium salt solution. Maximum absorbance for Be-CAS complex was performed atλmax 568 NM using acetate buffer at pH 4.6. Beer’s law was obeyed in the range from 0.02 to 9 ppm with molar absorptivityε = 0.22 × 104 mol−1 cm−1 and an average standard deviation of 0.7. The R.S.D for 10 replicate measurements of 1 ppm Be was 1.2%.

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