Question

What should be the tris hcl ph for extracion buffer

Answer 1

Answer:

above 6__ _☺☺

Answer 2

Answer:

Explanation:

⇒ Tris buffer , then your ph should be near neutral as it best maintains the GST function. 7.5 to 7.6. The following can work well: lysis buffer containing 50 mM Tris. HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Nonidet P-40, 5 mM Na3VO4, 20 mM NaF, 10 mM sodium pyrophosphate.

- how to adjust the pH of a tris HCI buffer

(1) Dissolve 121 g Tris base in 800 ml H2O.

(2) Adjust to desired pH with concentrated HCl. Approximately 70 ml HCl is needed to achieve a pH 7.4 solution, and 42 ml for a pH 8.0 solution.

(3) Adjust volume to 1 liter with H2O.

(4) Filter sterilize if necessary.

(5) Store up to 6 months at 4°C or room temperature.